Monday, January 14, 2013

Case of the Week 241

The following were seen in a stool specimen from a man with watery diarrhea.  The stain is a modified safranin stain.  They measure 8-10 microns in diameter.  Identification?

400x original magnification

1000x original magnification

Many thanks to Florida Fan for these beautiful images!

10 comments:

Scott C. said...

Blastocystis hominis

Anonymous said...

What's the calibration on the ocular micrometer -- what's the size of these objects?

Lionel said...

I am not sure of the size, but if its 4-5 ┬Ám, i would say Cryptosporium oocysts

Lionel said...

sorry for the misspelling, Cryptosporidium

Anonymous said...

Cyclospora...too big for Cryptosporidium...

Lee

Anonymous said...

The occular micrometer has been calibrated to measure:
1 graduation = 2.5 nm at 400x
1 graduation = 1.0 nm at 1000x
total magnification.

Florida fan

Anonymous said...

Cyclospora measure 8-10 um Cryptosporidium sp. 4-5 um

Hans

Parasite Gal said...

Shame on me! I've now added the size.

Unknown said...

Cyclospora cayetanensis, uniform safranin staining.

Anonymous said...

Just Curious...does anyone have an ocular micrometer that deviates from 100x = 10nm, 400x = 2.5 and 1000x = 1 nm. I have calibrated many scopes in my career and have never seen one different. Just Curious. Lee

ecollings@shoremedicalcenter.org