tag:blogger.com,1999:blog-629132641307534690.post510707921281422067..comments2024-03-26T16:04:11.096-05:00Comments on Creepy Dreadful Wonderful Parasites: Case of the Week 523ParasiteGalhttp://www.blogger.com/profile/09093150363550239544noreply@blogger.comBlogger16125tag:blogger.com,1999:blog-629132641307534690.post-13331205800423460182018-12-21T15:29:38.485-06:002018-12-21T15:29:38.485-06:00Indeed Blaine!
We tended to use the supernatant re...Indeed Blaine!<br />We tended to use the supernatant resulting from boiling bird seeds in water, called “eau de blé”. A perfect medium for growing acanthamoeba after it had been spiked with bacteria.Idzi P.noreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-26927283863665734272018-12-21T10:39:42.763-06:002018-12-21T10:39:42.763-06:00Well you can rule-out Balamuthia from the case nar...Well you can rule-out Balamuthia from the case narrative, because you cannot culture Balamuthia on bacteria. In nature, they feed on other free-living protozoa. In the lab you would need to culture them on cell lines.Blaine A. Mathisonnoreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-62798585163468663592018-12-20T21:02:37.583-06:002018-12-20T21:02:37.583-06:00Thank you one and allThank you one and allOld Onehttps://www.blogger.com/profile/03517349371972756602noreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-20380503534781112122018-12-20T19:46:37.535-06:002018-12-20T19:46:37.535-06:00Old one:
Naegleria fowleri:
Trophozoite Biphasic...Old one: <br /><br />Naegleria fowleri:<br />Trophozoite Biphasic (amebic) and flagellate forms; 8–15 (7–35) μm; lobate pseudopodia pseudopodia<br />(amebic form);<br />Cysts not present in tissue; small, smooth, rounded; 7–15 μm; <br />Require living cells (bacteria or cell culture); do not grow with >0.4% NaCl by 0.85% NaCl<br />Appearance in tissue: Smaller than Acanthamoeba spp.; dense endoplasm; less distinct nuclear staining <br /><br />Acanthamoeba spp.:<br />Trophozoite large (15–25 μm): no flagella; filiform pseudopodia (acanthopodia);<br />Cysts present in tissue; large with wrinkled double wall; <br />May grow without bacteria; not affected by 0.85% NaCl<br />Appearance in tissue: Large; rounded; less endoplasm; nucleus more distinct<br /><br />Balamuthia mandrillaris:<br />Trophozoites are usually irregular in shape, and actively feeding amebae may measure from 12 to 60 μm in length (normal 30 μm). Possesses a nucleus but may possess more than one nucleolus. In tissue culture, broad pseudopodia are usually seen; however, as the monolayer cells are destroyed, the trophozoites develop fingerlike pseudopodia:<br />Cysts are somewhat smaller than the trophozoites, are usually spherical, and measure from<br />13 to 30 μm in diameter, in EM are characterized by having three layers in the cyst wall: an outer wrinkled ectocyst, a middle structureless mesocyst, and an inner thin endocyst;<br />Shown not to grow well on E. coli-seeded nonnutrient agar plates, can be cultured in mammalian cell cultures;<br />Both trophozoites and cysts of B. mandrillaris are found in many of the same CNS tissues as are Acanthamoeba spp. B. mandrillaris may have more than one nucleolus in the nucleus in some tissue sections. Bernardino Rochanoreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-51356258905198836932018-12-20T19:27:58.969-06:002018-12-20T19:27:58.969-06:00Old One,
There is an excellent lecture on line giv...Old One,<br />There is an excellent lecture on line given by Dr. Visvesvara of CDC on the free living amoebas. If my memory is still correct, he named the amoeba Balamuthia in honor of his teacher Dr. Balamuth (just the same way Alexander Yersin named the Pateurella pestis, BTW Dr. Alexander Yersin was burried at the Suoi Dau facility of the Pasteur Institute in Vietnam during the Japanese occupation in World War II.)<br />Hope this help,<br />Florida FanAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-2116810166610948912018-12-20T15:52:55.797-06:002018-12-20T15:52:55.797-06:00Would it be possible for someone to review the mor...Would it be possible for someone to review the morphological features used to differentiate the cysts of Naegleria, Acanthamoeba, and Balamuthia ?Old Onehttps://www.blogger.com/profile/03517349371972756602noreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-20604128455154937022018-12-20T15:31:59.356-06:002018-12-20T15:31:59.356-06:00Definitely Acanthamoeba sp.
Cysts and trophozoites...Definitely Acanthamoeba sp.<br />Cysts and trophozoites.<br />I’ve seen it twice in the lab. Each time in lens recipients.<br />Too bad we can’t see the typical “exploding” pseudopoda here... <br />It is not unusual for this parasite to form cysts when growing in a culture medium.Idzi P.noreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-44369848255969972922018-12-20T10:13:09.056-06:002018-12-20T10:13:09.056-06:00Bilaminar cyst wall = Acanthamoeba.
Trilaminar cys...Bilaminar cyst wall = Acanthamoeba.<br />Trilaminar cyst wall = Balamuthia.<br /><br /><br />-HLCM fanAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-6520797089858003092018-12-19T10:05:42.637-06:002018-12-19T10:05:42.637-06:00Well, if not for the narrative I may not have imme...Well, if not for the narrative I may not have immediately recognized this as Acanthamoeba, since my experience with electron microscopy is limited to microsporidia. But the cyst wall morphology is c/w Acanthamoeba. Blaine A. Mathisonnoreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-66174153332709734152018-12-18T10:20:00.829-06:002018-12-18T10:20:00.829-06:00I would like to wish everyone a happy holiday seas...I would like to wish everyone a happy holiday season and extend a blessing we often see in our Christmas cards but I think it applies to most everyone.<br /><br />"Peace on earth and good will to all"<br /><br />Also to pass on a little seasonal parasite story.<br /><br />Many years ago about this time of year, I was asked to collect some Ascaris suum for class. The school was willing to pay milage for my car (a wonderful cash infusion for a needy technician). So I loaded the car and headed for the Hormel packing plant (creator and maker of Spam canned meat).<br /><br />Upon arrival I was met by a plant Supervisor who had me suit up with protective (for the meat not me) coverings. This included covering my face with head and beard nets. I looked like a real Dork (a total fool).<br /><br />The Supervisor walked me to the gutting floor where I met the Forman. I couldn't believe my eyes. Leaning back in his chair was a rotund man in white shirt and suspenders. His face round, his cheeks rosy, his full luxuriant white beard and hair- this could only be one person. In my mind I yelled, "Santa, it really is you.<br /><br />Santa looked at me and with a jolly Ho Ho Ho said " take that sh_t off your face".<br /><br />Needless to say the rest of the collection went well. Santa's elves did pull some gutting line humor, but it was all done in good spirits.<br /><br />I came away that day with three realizations.<br /><br />1. Minnesota hogs are loaded with ascarids.<br />2. My car made more money than I did.<br />3. Santa is alive and well in Austin, Minnesota.Old Onehttps://www.blogger.com/profile/03517349371972756602noreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-66225734648549795212018-12-18T03:10:51.889-06:002018-12-18T03:10:51.889-06:00Cisti e trofozoiti di Acanthamoeba spp.
Cisti e trofozoiti di Acanthamoeba spp.<br /><br />Sir Galahadhttps://www.blogger.com/profile/17629414661390580009noreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-90969788361260981692018-12-17T21:46:35.953-06:002018-12-17T21:46:35.953-06:00It never ceases to amaze me how quick and accurate...It never ceases to amaze me how quick and accurate everyone is with their identifications. I found this case to be very challenging.<br /><br />The specimens appear to be protozoan in both cyst and trophozoite forms. Having a single nuclei with a large centrally located karyosome. Many inclusion bodies present in cytoplasm, possibly food vacuoles (probably bacteria, consistent for a facultative parasite). Cross sections of cyst-forms show a bilaminar cyst wall (photos 1,3,4,5). The ectocyst wall is wrinkled, the endocyst wall is smoother by comparison. Endocyst and ectocyst come together and meet at ostioles (photos 4 and 5). These ostioles (pores) have an operculum inside the endocyst. These characteristics appear to be those of Acanthamoeba sp..<br /><br />I'd like to say "the eyes have it", but I may be wrong.Old Onehttps://www.blogger.com/profile/03517349371972756602noreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-24165691102540848352018-12-17T18:41:39.852-06:002018-12-17T18:41:39.852-06:00Trofozoitos, no son quistes, porque crecen en medi...Trofozoitos, no son quistes, porque crecen en medios con bacterias Luishttps://www.blogger.com/profile/01493940543449064698noreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-72795847465776093712018-12-17T13:39:19.594-06:002018-12-17T13:39:19.594-06:00Trophozoites and cysts of Acanthamoeba species.Trophozoites and cysts of Acanthamoeba species.Samhttps://www.blogger.com/profile/08527111856234825333noreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-75336307969835942722018-12-17T13:22:43.698-06:002018-12-17T13:22:43.698-06:00The clue is the liquid culture seeded with bacteri...The clue is the liquid culture seeded with bacteria. This clue points to an amoebic culture where the bacteria serve as food for the trophozoite stage of the amoeba. The polygonal appearance of the organism give us another clue compatible with Acanthamoeba sp. as observed through various stains.<br />Florida FanAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-629132641307534690.post-56253264622697047412018-12-17T12:54:32.422-06:002018-12-17T12:54:32.422-06:00It appears to be trophozoites and cyst of Acantham...It appears to be trophozoites and cyst of Acanthamoeba sp. Bernardino Rochanoreply@blogger.com