Monday, January 14, 2013

Case of the Week 241

The following were seen in a stool specimen from a man with watery diarrhea.  The stain is a modified safranin stain.  They measure 8-10 microns in diameter.  Identification?

400x original magnification

1000x original magnification

Many thanks to Florida Fan for these beautiful images!
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10 comments:

  1. Scott C.January 14, 2013 at 8:55 AM

    Blastocystis hominis

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  2. AnonymousJanuary 14, 2013 at 9:06 AM

    What's the calibration on the ocular micrometer -- what's the size of these objects?

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  3. LionelJanuary 14, 2013 at 9:55 AM

    I am not sure of the size, but if its 4-5 µm, i would say Cryptosporium oocysts

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  4. LionelJanuary 14, 2013 at 9:57 AM

    sorry for the misspelling, Cryptosporidium

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  5. AnonymousJanuary 14, 2013 at 11:38 AM

    Cyclospora...too big for Cryptosporidium...

    Lee

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  6. AnonymousJanuary 14, 2013 at 6:27 PM

    The occular micrometer has been calibrated to measure:
    1 graduation = 2.5 nm at 400x
    1 graduation = 1.0 nm at 1000x
    total magnification.

    Florida fan

    ReplyDelete
  7. AnonymousJanuary 15, 2013 at 8:49 AM

    Cyclospora measure 8-10 um Cryptosporidium sp. 4-5 um

    Hans

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  8. ParasiteGalJanuary 15, 2013 at 9:20 AM

    Shame on me! I've now added the size.

    ReplyDelete
  9. UnknownJanuary 15, 2013 at 2:00 PM

    Cyclospora cayetanensis, uniform safranin staining.

    ReplyDelete
  10. AnonymousJanuary 18, 2013 at 12:28 PM

    Just Curious...does anyone have an ocular micrometer that deviates from 100x = 10nm, 400x = 2.5 and 1000x = 1 nm. I have calibrated many scopes in my career and have never seen one different. Just Curious. Lee

    ecollings@shoremedicalcenter.org

    ReplyDelete