The following structures were seen in a Giemsa-stained peripheral blood smear from a southeast Asian immigrant. Identification? (CLICK ON IMAGES TO ENLARGE)
Microfilariae, and by the looks of morphology (sheathed, tapered tail, packed nuclei and a large distance between the last nuclei and end) and being from Asia, likely Brugia malayi.
First, this is a sheathed microfilaria. The second clue is that, among the sheathed microfilaria, Brugia malayii is the one whose sheath stains pink with Giemsa, Loa Loa and Wucheria bancrofti sheath does not stain with Giemsa. Other characteristics consistent with Brugia malayii I cludes: long empty cephalic space and the two terminal nuclei of the tail are well separated and they are very small (picture #3).
Microfilariae, and by the looks of morphology (sheathed, tapered tail, packed nuclei and a large distance between the last nuclei and end) and being from Asia, likely Brugia malayi.
ReplyDeleteLovely Brugia malayi.
ReplyDeleteFirst, this is a sheathed microfilaria. The second clue is that, among the sheathed microfilaria, Brugia malayii is the one whose sheath stains pink with Giemsa, Loa Loa and Wucheria bancrofti sheath does not stain with Giemsa. Other characteristics consistent with Brugia malayii I cludes: long empty cephalic space and the two terminal nuclei of the tail are well separated and they are very small (picture #3).
ReplyDeleteFlorida Fan
This comment has been removed by the author.
ReplyDeleteBrugia malayi - everyone above has explained very nicely why!
ReplyDeleteSheathed, tail tip nuclei, cephalic space longer therefore Brugia malayi
ReplyDeleteWe may add that Brugia timori's sheath does not stain with Giemsa and therefore can be excluded from the diagnosis.
ReplyDeleteFlorida Fan