Case of the Week 312

Dear Readers,
I'd like to first thank This Week in Parasitism and the hosts, Vincent Racaniello and Dickson Despommier for featuring my blog as their 75th podcast.  You can hear the podcast HERE. Please check out their other podcasts - they're fun and contain great information!

Now for our case this week:  A peripheral blood thin film from a patient with recurring fevers and recent travel to India.  Identification?

Case of the Week 311

Here's a nice straight-forward case this week.  The following were seen on Ova and Parasite exam performed on an adopted child from Ethiopia.  Identification? (CLICK ON THE IMAGES TO ENLARGE)

Answer to Case 311

Answer:  Ascaris lumbricoides and Trichuris trichiura eggs.
As noted by a few of the readers, both fertilized and unfertilized Ascaris ova are present.  It is common to see co-infections with geohelminths in patients from endemic settings. Therefore, a good take home point is:  if you see one parasite, keep looking!

Case of the week 310

I have an unusual case for you this week.  The following images are of a stool agar culture plate for Strongyloides stercoralis after 6 days of incubation at room temperature:

 

Using a standard light microscope, the following video was captured using the 10x objective (100x total magnification):

Note that there is subtle movement in these filamentous structures (the choppiness is an artifact of my video camera).

To further investigate this, we performed a Gram stain of material taken from the fringed edge:

Gram at 100x

 Gram at 1000x

Identification?  Suggested further work-up?

Answer to Case 310

Answer:  No Strongyloides stercoralis or other larvae detected.  A motile Bacillus sp. was present, accounting for the motility observed on the Strongyloides agar culture plate.

This was an unusual case because the bacteria produced an interesting motion which superficially resembled larval movement.  However, the observed motility was not the expected sinuous movement of larvae through bacteria, but instead, consisted of regular and continuous clockwise or counterclockwise swirling within a confined region of the plate.

Multiple Gram stained preparations from these areas showed large Gram-variable to Gram positive rods with spores consistent with a Bacillus species.  Smaller Gram negative bacilli (likely coliforms) were also seen in the background.

It's important to remember that things other than Strongyloides stercoralis larvae can demonstrate motility on Strongyloides agar cultures. In this case, the unusual motility pattern was due to fecal bacteria. However, we've also seen mites, hookworm larvae, and even larvae from free-living nematodes demonstrate visible motility.  That is why we work up all positive cultures with microscopic identification of the motile objects.

Thanks for the great discussion on this one!

Case of the Week 309

The following structures were seen in a refrigerated urine specimen.  Identification?

 

Answer to Case 309

Answer:  Schistosoma haematobium ova/eggs

Everyone who wrote in had the correct answer.  These eggs have a very characteristic appearance with a large size (112 - 170 micrometers in length) and terminal spine.  As mentioned by Hans, you can check to see if the eggs are still viable by looking for the internal beating flame cells, particularly in fresh specimens. Unfortunately this specimen was several days old by the time it had reached us (it had been sent in from another lab as a consult) and no motility was observed - possibly due to the age of the specimen.  Hans also mentions that you can hatch the eggs - that's always something fun to do!

Anon noted the considerable debris that was present in the background.  Indeed, one of the reasons I chose this case for a blog post is because it had a lot of crystals in the background which can make it challenging to spot the eggs. This is seen occasionally with refrigerated specimens; gently heating them will often dissolve the crystals and allow the urine to be examined for parasite eggs.  Some types of urine crystals are commonly seen and do not necessarily indicate disease.  However, other types of crystals are strongly associated with disease - particularly when persistent.  When in doubt, consult your Clinical Chemist or suggest that a urine specimen be sent for crystal analysis.