Case of the Week 294

The following were seen in a stool ova and parasite exam in a 34 year old businessman with recent travel to India.  He reports a 2-week history of watery diarrhea.  They are relatively few in number and range in size from 8 to 20 micrometers in diameter.  How would you interpret this finding?

Case of the Week 293

Several hairs from the groin region of a 24 year old male were submitted to the Clinical Parasitology lab for examination.  One hair contained the following structure cemented onto the hair shaft.  It measures approximately 2 mm in length.  Identification?

Answer to Case 293

Answer:  nit (egg) of Pthirus pubis, the crab louse

You can differentiate this nit from the similarly appearing nits of the head and body lice by its taller operculum (cone of cells at the apex):

And now a poem from Blaine Mathison (I'm not taking responsibility for this one!)

Chilling at home in his bachelor pad

trying to get all the booty that he could grab

but his latest of trysts

gave him a gift that persists

for now he's host to Sebastian the 'Crab'

Case of the Week 292

The following is a skin biopsy from an ulcerating lesion on the left thigh of a 70 year old woman.  She is being treated with chemotherapy for diffuse large B cell lymphoma.

Hematoxylin and Eosin (H&E), 20x magnification 

H&E, 40x

H&E, 400x

H&E, 400x

H&E, 1000x

Gomori Methenamine Silver (GMS), 1000x

Answer to Case 292

Answer:  Trophozoites and Cysts of Acanthamoeba species/Balamuthia mandrillaris 

A multiplex PCR for free-living amebae was also performed on this tissue, which was positive for Acanthamoeba sp. DNA.

Many of you noted that the organism morphology is characteristic for Acanthamoeba/Balamuthia (it is nearly impossible to tell the 2 apart by light microscopy, so I group them together) but mentioned that the skin is an unusual location for these organisms.  It is correct that we don't usually see the free-living amebae in the skin.  However, if you take a look at the CDC life cycle of Acanthamoeba and B. mandrillaris, you'll note that one port of entry to the blood stream (and eventually the brain) is broken skin.  Therefore, it is essential to identify amebae in skin biopsies so that treatment can be implemented before dissemination occurs.  In this case, note how the organisms are clustered around blood vessels, indicating their potential for hematogenous dissemination!  Fortunately this patient did not have evidence of brain involvement.

Case of the Week 291

The following was seen in a Giemsa-stained thick peripheral blood film from an immigrant from Congo.  The patient reports focal swelling and tenderness in his left arm, but is otherwise asymptomatic.

(shown at 1000x magnification

Answer to Case 291

Answer:  Loa loa

The key features that aid in identification are the presence of a non-staining sheath (below, arrows), short head space and tapered tail with irregularly-spaced nuclei extending to the tip of the tail (below, arrowhead).