Case of the Week 745

We have moved onto microfilariae found in SKIN SNIPS - what fun! There are only a couple to choose from. Can you tell which one this is? The microfilariae are approximately 200 micrometers long.

Carazzi stain

Giemsa stain:

Case of the Week 744

 We are in the home stretch for the microfilariae! Can you believe that we have just a few more to go? (Unless I decide to repeat some 😉)  This week's case shows microfilariae that are approximately 200 micrometers long. The patient is a resident of Brazil and has moderate peripheral eosinophilia. He is otherwise asymptomatic. 

Carazzi stain (Knott's concentration):

Giemsa stain (thick blood film):

Answer to Case 744

 Answer to the Parasite Case of the Week 744: Mansonella perstans microfilariae.

As described by Florida Fan, "This is a rather small microfilaria, its width is only about half the diameter of the surrounding neutrophils. The Carrazi stain [a hematoxylin-based stain] did not show a sheath either. As such, we can definitely rule out all the sheathed and large microfilaria. We know that we are dealing with Mansonella species. The tail of this Mansonella is not curved , this allows us to eliminate Mansonella streptocerca (strepto = curved, cerca = tail) [and also the source is not tissue]. The tail is also not pointed, this rules out Mansonella ozzardi. We only have one left with a blunt tail: Mansonella perstans which persists." 

This image nicely shows all of these features:

You have all done a great job learning to differentiate the small, unsheathed blood microfilariae (i.e. Mansonella perstans and M. ozzardi) from the larger, sheathed microfilariae. 

Of course, co-infections can occur, and the following is a stunning photograph of M. perstans and Loa loa co-infection. I think we can all appreciated that the Loa loa microfilaria is the top based on its larger size and sheath. 

Thank you for these outstanding cases, Idzi!

Next week we will finish up with the tissue microfilariae. Will you be able to tell them apart?

Parasite Case of the Week 743

Welcome back for more microfilariae from Idzi Potters and the Institute of Tropical Medicine, Antwerp. The following microfilariae were seen in Giemsa-stained thick blood films from a man living on Alor Island, Indonesia. They measure approximately 305-315 micrometers in length. 

What is your identification? What is your primary differential?

Answer to Case 743

 Answer to the Parasite Case of the Week 743: Brugia timori microfilariae. This was one of the tougher cases, but everyone did a great job narrowing the differential to Brugia. As noted by Florida Fan and Anonymous, we can immediately rule out Mansonella species based on the small size (length and diameter) of the microfilariae. Florida Fan also notes that the sheath is visible, confirming that we are dealing with Loa loa, Wuchereria bancrofti or the Brugia genus. 

He then details his method for coming to an exact diagnosis: "First the column of nuclei is so compact that we can rule out Wuchereria bancrofti, also the nuclei go to the end of the tail. Yet, the two terminal nuclei are distinctly separated from the nuclei column, this rules out any chance of being Loa loa. Now we have the Brugia genus left, but where is the pink sheath pertinent to Brugia malayi when stained with Giemsa stain? As such, we eliminate Brugia malayi. By the elimination process, we’re left with its cousin Brugia timori, the sheath of this one does not stain pink with Giemsa stain. Now let’s check the map of the lesser Sundae island, the Alor island is right North of Timor."

I will also point out that the head space is longer than that of B. malayi and there are a larger number of single-file nuclei going towards the tail. These features are more subtle and can be difficult to appreciate. I've done my best to point out some of them in the image below.

Thank you all for sticking with us on our microfilariae journey. The microfilariae can be very challenging to identify, and are rare in many parts of the world, so our lab staff may not have the opportunity to examine these organisms outside of their EQA/PT schema. 

 

Case of the Week 742

This week's case features another beautiful example of microfilariae in blood. The patient is from sub-Saharan Africa and presents with chronic swelling of his left leg. The microfilariae measure approximately 270 µm in length. 

Carazzi stain (Knott's concentration):

Giemsa Stain (thick blood film)

What is your identification? What time should blood be collected for this examination?

Answer to Case 742

 Answer to the Parasite Case of the Week 742: Wuchereria bancrofti microfilariae.

I really enjoyed reading the comments on this case. FloridaFan provided this excellent description of his approach to microfilariae identification: "First the width of the worm is about the same as that of the surrounding neutrophils. Second, its length is greater than 200 micrometers. Third, it has a sheath. Now we know we are dealing with Loa loa, Wuchereria, or the Brugia ones, not with any little pesky Brugia. The next consideration is that the column of nuclei is continuous, the terminal nuclei are not separate from the immediate anterior nuclei. This eliminates the possibility of the Brugia malayi and timori, over that these are “far fetched” geographically. We are left with two candidates Loa loa and Wuchereria bancrofti. Though the tail is not so obvious, the Carrazi stain did show that the nuclei column terminates well short from the end of the tail. This rules out Loa loa. The only culprit left is Wuchereria bancrofti.

An anonymous commenter also noted that "Its nuclear column is relatively loose, and individual nuclei can be visualized throughout the column. The sheath is does not stain pink with Giemsa stain (as in Brugia malayi)."

These features can be nicely visualized in the photo of the Carazzi-stained Knott's concentration. Note that the Carazzi stain is a hematoxylin-based stain (rather than conventional Giemsa) that helps to demonstrate the sheath. It is a very useful stain to have!

Thanks again to Idzi Potters and the Institute of Tropical Medicine, Anwerp, for this beautiful case.

Case of the Week 741

Wow, we are already on our 3rd filarial case! The following lovely case from Idzi Potters and the Institute of Tropical Medicine, Antwerp, is another microfilaria found in blood. 

The patient is a middle aged male farmer from Central America who was noted to have mild eosinophilia on routine complete blood count. He is otherwise asymptomatic. The microfilariae measure approximately 175 micrometers in length. What is your identification?

Answer to Case 741

 Answer to the Parasite Case of the Week 741: Mansonella ozzardi

As many of you noted, we can get to an identification of Mansonella sp. by the small size (length of just 175 micrometers, and the width less than the surrounding white blood cells). There is also no visible sheath, which is supportive of the diagnosis. 

So the next question is - which Mansonella is present?? As FloridaFan mentioned, the tip of the tail is pointed rather than blunt, which leads us away from M. perstans. Also, the source is not skin snips, so M. streptocerca is unlikely. That leaves M. ozzardi by default. 

To confirm this, we would look at the tail to see if the nuclei go to the tip (M. perstans) or not (M. ozzardi). It's a bit hard to tell in this case, but if you look closely, you can see that the nuclei DON'T go to the tip of the tail. So - Mansonella ozzardi, it is!

Congratulations to those of you who were able to get to Mansonella species. The microfilariae can be hard to identify, so this is definitely advanced  parasitology!

Case of the Week 740

Welcome to our next filarial case by Idzi Potters and the Institute of Tropical Medicine, Antwerp. This week features the following lovely microfilariae seen in a Giemsa-stained thick blood film. They measure approximately 220 micrometers in length. Identification?