A 30 yo man with travel to South America presented with chronic non-healing warty skin lesions. Repeated skin biopsies were negative for microorganisms by histopathology but culture using brain-heart infusion media with rabbit blood grew the following on day 12 of incubation at 25C.
(1000x original magnification)
(1000x original magnification, Giemsa)
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEi9_n_1HwJ8ZE2qemOuBM8Xu6fX289dXzEg07Khbel_RqIA3XHyBkCWDO49cgDZLOhRV5r22Gn-6Uhbzpb6WtPTqx8s_0pvuIdDLM19h_CbDcDiK_lsb9n3sCUP-zC7vLmdo-0pSpVuVqFb/s400/COW1.jpg)
![](https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEgWgNIeH0Gh8B8491ug86aSN-shYshgahlYRcHwq9LK6ZWt6s0o2pchLPr5_yg5i6tgpXc6sKdZdDihOuSF4OuNymTyWEjO-FL479Lk9BrKJcYKLs27WOiRsGYE94ZTrykKW9Xe-ZX8Y6Bs/s400/COW2.jpg)
Diagnosis?
6 comments:
could it be Trypanosoma cruzi
Leishmania promastigotes; the amastigote is seen in tissue, but they convert in culture to promastigotes.
With "chronic non-healing warty skin lesions" I would say Leishmania sp.
Leishmania - at first I thought the skin lesions could be a reation to the bite of Reduviid bugs and thus, that it could be T. cruzi, but if the lesions are chronic, then I agree with the others above and say that these are Leishmania promastigotes ...
Gotta be cutaneous Leishmaniasis > New World; speciation to follow! It is really cool that we can perform the cultures in our lab now. Be nice if we can speciate - though likely will need to rely on the CDC Leishmania for that. Very Cool! - Mundele Djo
the faint big darkpink blob is the nucleus.
The small clear pink dot is the kinetoplast
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