The following were seen in a stool specimen from a man with watery diarrhea. The stain is a modified safranin stain. They measure 8-10 microns in diameter. Identification?
400x original magnification
1000x original magnification
Many thanks to Florida Fan for these beautiful images!
Just Curious...does anyone have an ocular micrometer that deviates from 100x = 10nm, 400x = 2.5 and 1000x = 1 nm. I have calibrated many scopes in my career and have never seen one different. Just Curious. Lee
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10 comments:
Blastocystis hominis
What's the calibration on the ocular micrometer -- what's the size of these objects?
I am not sure of the size, but if its 4-5 µm, i would say Cryptosporium oocysts
sorry for the misspelling, Cryptosporidium
Cyclospora...too big for Cryptosporidium...
Lee
The occular micrometer has been calibrated to measure:
1 graduation = 2.5 nm at 400x
1 graduation = 1.0 nm at 1000x
total magnification.
Florida fan
Cyclospora measure 8-10 um Cryptosporidium sp. 4-5 um
Hans
Shame on me! I've now added the size.
Cyclospora cayetanensis, uniform safranin staining.
Just Curious...does anyone have an ocular micrometer that deviates from 100x = 10nm, 400x = 2.5 and 1000x = 1 nm. I have calibrated many scopes in my career and have never seen one different. Just Curious. Lee
ecollings@shoremedicalcenter.org
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