The following were seen in Giemsa-stained peripheral blood films from an Indonesian man. Identification?
7 comments:
Anonymous
said...
Brugia malayi.
Average approximate length 200-250µm, stained sheath (which differentiates it from B. timori) tail is tapered with a gap between the two terminal nuclei.
Agree with all answers so far. First, the sheath stains pink with Giemsa and this separates these microfilaria from W. bancrofti, Loa loa, and B. timori. The lack of a single file caudal nuclei is indicative of B. malayi. The pictures clearly show the two terminal nuclei, a characteristic pertinent to B. malayi. Finally the origin of the patient is consistent with the geographic distribution of B. malayi.
Sheathed microfilaria can be remembered as follows - Wears Long Britches for Wuchereria, Brugia and Loa Loa. As for tail nuclei - Wuchereria is Without, Brugia has Bi (two) nuclei and Loa Loa nuclei 'flowa-flowa' to the tip.
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7 comments:
Brugia malayi.
Average approximate length 200-250µm, stained sheath (which differentiates it from B. timori) tail is tapered with a gap between the two terminal nuclei.
Agree.
Agree with all answers so far. First, the sheath stains pink with Giemsa and this separates these microfilaria from W. bancrofti, Loa loa, and B. timori. The lack of a single file caudal nuclei is indicative of B. malayi. The pictures clearly show the two terminal nuclei, a characteristic pertinent to B. malayi. Finally the origin of the patient is consistent with the geographic distribution of B. malayi.
Florida Fan
Agree with Brugia malayi. Sheath discounts Mansonella and tail morphology suggests it is not Wuchereria or Loa.
The sheath and 2 terminal nuclei did it for me. B. malayi!
Lee
Agree with Brugia malayi.
Wan Hafiz
My favorite Dr. Pritt lesson:
Sheathed microfilaria can be remembered as follows - Wears Long Britches for Wuchereria, Brugia and Loa Loa. As for tail nuclei - Wuchereria is Without, Brugia has Bi (two) nuclei and Loa Loa nuclei 'flowa-flowa' to the tip.
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