This week's case was generously donated by Dr. Nazia Nagi in New Delhi, India. She saw these 'cute' little objects (around 15 micrometers long) during her rotation in the Diarrhoeal Laboratory. The patient is a young teenager with diarrhea lasting for 15-17 days. Identification?
Saline wet mounts:
Iodine wet mount:
9 comments:
Beautiful autumn leaves falling in the wind in a sliding side to side motion. The morphological most beautiful of all trophozoites Giardia lamblia. The cysts show characteristic axiostyle, chromatoidal bodies in some and nuclei. So familiar yet depending on the orientation, Giardia trophozoite and cysts can present a little challenge to parasitologists at times. Most of us are used to see the typical kite shaped trophozoite with its nuclei, sucking disks and flowing flagella’s. When these trophozoites turn side way, we may see only ạ leafy profile, and when they stand on their tails they will look like the kid next door poking his head over the privacy fence showing only the top of the head and the two eyes. The typical ovoid cysts can float on their ends and we may observe only a spherical object with a few discernible dots for nuclei. In my practicing days, I built models of both the trophozoite and the cyst and rotated them around to show the team the different morphology when viewed at different angles. Beautiful case indeed.
Florida Fan
Trophozoites and cyst of Giardia lamblia... beautiful
On a final thought, the poor patient must have drunk “beaver water” though the hard working rodent may not be the culprit to blame.
Florida Fan.
I love your description of the morphological aspects of Giardia, beautifully said, 100 % right !
Sure !
Smail Belazzoug
Those are Giardia intestinalis cysts and trophozoites.
The hallmark for detecting these strructures, especially cysts, in unstained wet mount is the presence of a longitudinal axoneme inside each, while nuclei are usually difficult to see and count
Giardia intestinalis cysts and trophozoites
Cysts and trophozoites of Giardia duodenalis (syn. Giardia lamblia, Giardia intestinalis).
Beautiful pictures.
And "cute" indeed. Especially the trophozoites when one can see their "falling-leaf-in-autumn"-motion in a freshly produced diarrheal sample.
Off Topic:
https://www.theguardian.com/australia-news/2023/aug/28/live-worm-living-womans-brain-australia-depression-forgetfulness
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